Modified inverse PCR method for cloning the flanking sequences from human cell pools

Biotechniques. 1999 Oct;27(4):660-2. doi: 10.2144/99274bm05.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular*
  • DNA / chemistry*
  • DNA Primers
  • DNA-Binding Proteins / genetics
  • Electrophoresis, Agar Gel
  • Genome, Human*
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Taq Polymerase
  • Transposases / genetics

Substances

  • DNA Primers
  • DNA-Binding Proteins
  • DNA
  • Taq Polymerase
  • Tc1 transposase
  • Transposases