Abstract
The majority of the known Ly49 family members have been isolated from either C57BL/6 (B6) or BALB/c mice. Interestingly, the anti-Ly49 Ab reactivities observed in 129/J mice are different from those of B6 mice. Furthermore, immunoprecipitation of 129/J NK cell lysates with YE1/32 and YE1/48, Abs specific for the inhibitory Ly49A in B6, resulted in detection of the activation-associated DAP12 molecule. These results indicated a need for a more detailed study of this strain. Therefore, a cloning strategy was devised to isolate Ly49 cDNAs from 129/J mice. An immunoreceptor tyrosine-based inhibitory motif-containing, Ly49D-related clone was discovered that we have named Ly49O, and one immunoreceptor tyrosine-based inhibitory motif-lacking, Ly49A-related clone was discovered that we have named Ly49P. No anti-Ly49 mAb reacted with Ly49O, whereas the molecule encoded by the Ly49P cDNA was found to react with YE1/32 and YE1/48. Ly49P was found to associate with mouse DAP12, and Ab-mediated cross-linking of Ly49P resulted in mouse DAP12 phosphorylation and Ca2+ mobilization, indicating that Ly49P is a competent activation receptor. Ly49P, therefore, represents a novel member of the Ly49 activating receptor subfamily.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adaptor Proteins, Signal Transducing
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / metabolism
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Antigen-Antibody Reactions
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Antigens, Ly / chemistry
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Antigens, Ly / genetics*
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Antigens, Ly / immunology
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Antigens, Ly / metabolism
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Base Sequence
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Calcium Signaling / immunology
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Carrier Proteins / genetics*
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Cloning, Molecular
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DNA, Complementary / isolation & purification
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Humans
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Killer Cells, Natural / immunology
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Killer Cells, Natural / metabolism
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Lectins, C-Type
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Lymphocyte Activation / immunology*
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Membrane Proteins / genetics*
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Mice
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Mice, Inbred Strains
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Molecular Sequence Data
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NK Cell Lectin-Like Receptor Subfamily A
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Phosphorylation
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Polymerase Chain Reaction
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Receptors, Immunologic / chemistry
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Receptors, Immunologic / genetics*
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Receptors, Immunologic / immunology
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Receptors, Immunologic / metabolism
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Receptors, Immunologic / physiology
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Receptors, NK Cell Lectin-Like
Substances
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Adaptor Proteins, Signal Transducing
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Antibodies, Monoclonal
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Antigens, Ly
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Carrier Proteins
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DNA, Complementary
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Klra1 protein, mouse
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Klra4 protein, mouse
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Lectins, C-Type
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Ly49O receptor
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Ly49P receptor
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Membrane Proteins
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NK Cell Lectin-Like Receptor Subfamily A
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Receptors, Immunologic
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Receptors, NK Cell Lectin-Like
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TYROBP protein, human
Associated data
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GENBANK/AF146570
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GENBANK/AF146571