Sexual transmission and propagation of SIV and HIV in resting and activated CD4+ T cells

Science. 1999 Nov 12;286(5443):1353-7. doi: 10.1126/science.286.5443.1353.

Abstract

In sexual transmission of simian immunodeficiency virus, and early and later stages of human immunodeficiency virus-type 1 (HIV-1) infection, both viruses were found to replicate predominantly in CD4(+) T cells at the portal of entry and in lymphoid tissues. Infection was propagated not only in activated and proliferating T cells but also, surprisingly, in resting T cells. The infected proliferating cells correspond to the short-lived population that produces the bulk of HIV-1. Most of the HIV-1-infected resting T cells persisted after antiretroviral therapy. Latently and chronically infected cells that may be derived from this population pose challenges to eradicating infection and developing an effective vaccine.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Anti-HIV Agents / therapeutic use
  • CD4-Positive T-Lymphocytes / cytology
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / virology*
  • Cell Cycle
  • Cervix Uteri / virology
  • Epithelial Cells / virology
  • Female
  • HIV Infections / drug therapy
  • HIV Infections / transmission*
  • HIV Infections / virology
  • HIV-1 / physiology*
  • Lymph Nodes / virology
  • Lymphocyte Activation*
  • Macaca mulatta
  • RNA, Viral / analysis
  • Simian Acquired Immunodeficiency Syndrome / transmission*
  • Simian Acquired Immunodeficiency Syndrome / virology
  • Simian Immunodeficiency Virus / physiology*
  • Time Factors
  • Virus Replication

Substances

  • Anti-HIV Agents
  • RNA, Viral