Dependence of echovirus 9 on the enterovirus RNA replication inhibitor 2-(alpha-Hydroxybenzyl)-benzimidazole maps to nonstructural protein 2C

D Hadaschik; M Klein; H Zimmermann; H J Eggers; B Nelsen-Salz

doi:10.1128/JVI.73.12.10536-10539.1999

Dependence of echovirus 9 on the enterovirus RNA replication inhibitor 2-(alpha-Hydroxybenzyl)-benzimidazole maps to nonstructural protein 2C

J Virol. 1999 Dec;73(12):10536-9. doi: 10.1128/JVI.73.12.10536-10539.1999.

Authors

D Hadaschik¹, M Klein, H Zimmermann, H J Eggers, B Nelsen-Salz

Affiliation

¹ Institut für Virologie der Universität zu Köln, 50935 Cologne, Germany.

Abstract

HBB [2-(alpha-hydroxybenzyl)-benzimidazole] selectively inhibits RNA synthesis of most enteroviruses. However, isolation of HBB-dependent variants is possible. Sequence analysis and characterization of recombinant viruses revealed that HBB dependence maps to the nonstructural protein 2C. A single point mutation at position C(4782)U is sufficient to establish the HBB-dependent phenotype in our echovirus 9 model.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antiviral Agents / pharmacology*
Benzimidazoles / pharmacology*
Cloning, Molecular
Enterovirus B, Human / drug effects*
Enterovirus B, Human / enzymology
Enterovirus B, Human / genetics
Enterovirus B, Human / physiology
Humans
Point Mutation
RNA Helicases / genetics*
RNA, Viral / biosynthesis
Viral Proteins

Substances

Antiviral Agents
Benzimidazoles
RNA, Viral
Viral Proteins
HBBPC
2C helicase
RNA Helicases