Abstract
Here we show that the Drosophila homologue of Lissencephaly-1, DLis-1, acts together with Bicaudal-D (Bic-D), Egalitarian (Egl), dynein and microtubules to determine oocyte identity. DLis-1 is further required for nurse-cell-to-oocyte transport during oocyte growth, and for the positioning of the nucleus in the oocyte. Immunostaining of DLis-1 protein reveals a cortical localization that is independent of microtubules. DLis-1 may function in this position as a cortical anchor for the other nuclear-localization factors. DLis-1 and Bic-D are further required for nuclear localization in the developing nervous system, indicating that homologues of Bic-D, dynein and Egl-like proteins may also be involved in vertebrate neural migration and that their absence may cause a Miller-Dieker-like lissencephaly.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Nucleus / metabolism*
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Drosophila Proteins*
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Drosophila melanogaster / embryology
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Drosophila melanogaster / genetics*
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Drosophila melanogaster / growth & development
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Drosophila melanogaster / metabolism
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Dyneins / metabolism*
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Enhancer Elements, Genetic / genetics
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Insect Proteins / metabolism*
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Microscopy, Fluorescence
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Microtubule-Associated Proteins / genetics
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Microtubule-Associated Proteins / metabolism*
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Microtubules / metabolism
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Molecular Sequence Data
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Neurons / physiology
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Oocytes / physiology*
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Oogenesis / genetics
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Oogenesis / physiology
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Photoreceptor Cells, Invertebrate / physiology
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Sequence Alignment
Substances
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BicD protein, Drosophila
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Drosophila Proteins
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Insect Proteins
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Lis-1 protein, Drosophila
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Microtubule-Associated Proteins
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egl protein, Drosophila
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Dyneins