Migration of CD18-deficient neutrophils in vitro: evidence for a CD18-independent pathway induced by IL-8

Biochim Biophys Acta. 2000 Jan 3;1500(1):70-6. doi: 10.1016/s0925-4439(99)00089-7.

Abstract

Neutrophils isolated from a child with severe leukocyte adhesion deficiency 1 (LAD1) had a complete absence of expression of the CD11/CD18 beta2 integrin family of adhesion molecules, and were shown to be deficient in the in vitro adhesion and migration properties. However, we found that interleukin-8 (IL8), a potent chemoattractant for neutrophils, and sputum sol phase induced these LAD1 neutrophils to migrate through an endothelial cell layer in vitro, and confirmed that this migration was CD18-independent. These findings add to evidence of CD18-independent mechanisms of neutrophil recruitment, in particular neutrophil infiltration into the lungs, where IL8 may be an important recruitment factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / metabolism
  • CD11 Antigens / immunology
  • CD18 Antigens / immunology*
  • Cell Adhesion / genetics
  • Cell Movement / drug effects*
  • Cell Movement / genetics
  • Cells, Cultured
  • Diffusion Chambers, Culture
  • Endothelium, Vascular / cytology
  • Flow Cytometry
  • Humans
  • Infant
  • Integrin alphaXbeta2
  • Interleukin-8 / pharmacology*
  • Leukocyte-Adhesion Deficiency Syndrome / immunology*
  • Leukocyte-Adhesion Deficiency Syndrome / pathology
  • Lymphocyte Function-Associated Antigen-1 / biosynthesis
  • Macrophage-1 Antigen / biosynthesis
  • Male
  • Membrane Glycoproteins / biosynthesis
  • Neutrophil Infiltration / drug effects
  • Neutrophils / cytology
  • Neutrophils / drug effects*
  • Neutrophils / immunology
  • Precipitin Tests

Substances

  • Antibodies
  • CD11 Antigens
  • CD18 Antigens
  • Integrin alphaXbeta2
  • Interleukin-8
  • Lymphocyte Function-Associated Antigen-1
  • Macrophage-1 Antigen
  • Membrane Glycoproteins