Abstract
SYBL1 is a gene in the 320kb human pseudo-autosomal region at the terminus of Xq and Yq. In contrast to other pseudoautosomal genes, SYBL1 is inactivated on one X in every female cell, and is also inactive on the Y of male cells. Hypermethylation of the CpG island associated with the human gene is involved in this phenomenon. In an attempt to further examine its regulation, the genomic organization of the X-linked mouse Sybl1 homolog was analyzed and compared with the human gene. Human and mouse show the same exon number, exon-intron junctions and a highly conserved basal promoter. The structural and functional conservation of basal regulatory regions suggests that inactivation is imposed by similar auxiliary epistatic regulatory mechanism.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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Blotting, Northern
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Chloramphenicol O-Acetyltransferase / genetics
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Chloramphenicol O-Acetyltransferase / metabolism
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DNA / chemistry
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DNA / genetics
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Exons
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Gene Expression
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Gene Expression Regulation, Developmental
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Genes / genetics*
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HeLa Cells
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Humans
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Introns
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Male
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Membrane Proteins / genetics*
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Mice
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Molecular Sequence Data
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Promoter Regions, Genetic / genetics
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R-SNARE Proteins
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Regulatory Sequences, Nucleic Acid / genetics
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid
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Tissue Distribution
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Transcription, Genetic
Substances
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Membrane Proteins
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R-SNARE Proteins
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RNA, Messenger
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Recombinant Fusion Proteins
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Sybl1 protein, mouse
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VAMP7 protein, human
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DNA
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Chloramphenicol O-Acetyltransferase