Abstract
A gene fusion system based on plasmid pBBR1MCS and the expression of green fluorescent protein was developed for Brucella suis, allowing isolation of constitutive and inducible genes. Bacteria containing promoter fusions of chromosomal DNA to gfp were visualized by fluorescence microscopy and examined by flow cytometry. Twelve clones containing gene fragments induced inside J774 murine macrophages were isolated and further characterized.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Brucella / genetics*
-
Brucella / growth & development
-
Brucella / metabolism
-
Cell Line
-
Gene Expression Regulation, Bacterial*
-
Gene Expression*
-
Genes, Reporter
-
Green Fluorescent Proteins
-
Luminescent Proteins / biosynthesis
-
Luminescent Proteins / genetics*
-
Macrophages / microbiology
-
Mice
-
Plasmids / genetics
-
Promoter Regions, Genetic
-
Recombinant Fusion Proteins / biosynthesis
Substances
-
Luminescent Proteins
-
Recombinant Fusion Proteins
-
Green Fluorescent Proteins