The use of carboxyfluorescein diacetate succinimidyl ester to determine the site, duration and cell type responsible for antigen presentation in vivo

Immunol Cell Biol. 1999 Dec;77(6):539-43. doi: 10.1046/j.1440-1711.1999.00868.x.

Abstract

This report examines the use of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) to determine the site, duration and cell type responsible for antigen presentation in vivo. Evidence that CFSE-labelled T cells can be used to determine where various types of antigens are presented, including auto-antigens, oral antigens and cell-associated foreign antigens, is provided. Using this technique, the length of time antigen is presented after acquisition by APC was measured. Finally, CFSE labelling was used to identify the origin of the APC responsible for different forms of antigen presentation.

Publication types

  • Review

MeSH terms

  • Animals
  • Antigen Presentation / immunology*
  • Antigen-Presenting Cells / cytology
  • Antigen-Presenting Cells / immunology*
  • Autoantigens / immunology
  • CD8-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / immunology
  • Cell Division / immunology
  • Fluoresceins*
  • Fluorescent Dyes*
  • Immune Tolerance / immunology
  • Islets of Langerhans / cytology
  • Islets of Langerhans / immunology
  • Lymph Nodes / cytology
  • Lymph Nodes / immunology
  • Mice
  • Mice, Transgenic
  • Spleen / cytology
  • Spleen / immunology
  • Succinimides*

Substances

  • 5-(6)-carboxyfluorescein diacetate succinimidyl ester
  • Autoantigens
  • Fluoresceins
  • Fluorescent Dyes
  • Succinimides