Electrophysiological evidence for a hyperpolarizing, galanin (1-15)-selective receptor on hippocampal CA3 pyramidal neurons

Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14583-7. doi: 10.1073/pnas.96.25.14583.

Abstract

The effects of the 29-amino acid neuropeptide galanin [GAL (1-29)], GAL(1-15), GAL(1-16), and the GAL subtype 2 receptor agonist D-tryptophan(2)-GAL(1-29) were studied in the dorsal hippocampus in vitro with intracellular recording techniques. GAL(1-15) induced, in the presence of tetrodotoxin, a dose-dependent hyperpolarization in hippocampal CA3 neurons. Most of the GAL(1-15)-sensitive neurons did not respond to GAL(1-29), GAL(1-16), or D-tryptophan(2)-GAL(1-29). These results indicate the presence of a distinct, yet-to-be cloned GAL(1-15)-selective receptor on CA3 neurons in the dorsal hippocampus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Galanin / pharmacology*
  • Hippocampus / chemistry
  • Hippocampus / drug effects*
  • Hippocampus / physiology
  • Male
  • Membrane Potentials / drug effects
  • Peptide Fragments / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Galanin
  • Receptors, Neuropeptide / analysis*

Substances

  • Peptide Fragments
  • Receptors, Galanin
  • Receptors, Neuropeptide
  • galanin (1-15)
  • galanin (1-16)
  • Galanin