Abstract
TBP (TATA-binding protein)-associated factors (TAF(II)s) are components of large multiprotein complexes such as TFIID, TFTC, STAGA, PCAF/GCN5, and SAGA, which play a key role in the regulation of gene expression by RNA polymerase II. The structures of TFIID and TFTC have been determined at 3.5-nanometer resolution by electron microscopy and digital image analysis of single particles. Human TFIID resembles a macromolecular clamp that contains four globular domains organized around a solvent-accessible groove of a size suitable to bind DNA. TFTC is larger and contains five domains, four of which are similar to TFIID.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA-Binding Proteins / analysis
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DNA-Binding Proteins / chemistry*
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HeLa Cells
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Humans
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Image Processing, Computer-Assisted
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Microscopy, Electron
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Models, Molecular*
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Protein Conformation
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Protein Folding
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Protein Structure, Tertiary
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TATA-Binding Protein Associated Factors*
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Transcription Factor TFIID
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Transcription Factors / analysis
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Transcription Factors / chemistry*
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Transcription Factors, TFII / chemistry*
Substances
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DNA-Binding Proteins
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TAF10 protein, human
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TATA-Binding Protein Associated Factors
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Transcription Factor TFIID
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Transcription Factors
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Transcription Factors, TFII