Effect of anti-ICAM-1 on bronchial response: bronchoalveolar lavage fluid (BALF) and ultrastructural changes of bronchial epithelium in guinea pigs with dual phase bronchial response

Methods Find Exp Clin Pharmacol. 1999 Oct;21(8):541-7. doi: 10.1358/mf.1999.21.8.794836.

Abstract

Eosinophils play an important role in the development of bronchial asthma, and the association between ICAM-1 and activation and migration of local eosinophils is attracting attention. Using an asthmatic model of dual phase bronchial response, the effects of anti-ICAM-1 antibody on the airway resistance, cell composition in the bronchoalveolar lavage fluid (BALF) and ultrastructure of bronchial ciliated epithelium were examined under the provoked response by inhalation of the antigen. By administration of anti-ICAM-1 antibody, the late asthmatic response (LAR) was suppressed. In the examination of bronchoalveolar lavage fluid, a significant decrease in eosinophils was found in LAR. In examining transmission and scanning electron microscopies, no difference was found in the immediate asthmatic response, but marked suppression of deciduation of bronchial ciliated epithelium was observed in LAR. These results indicated that anti-ICAM-1 antibody suppressed bronchial asthmatic attack, mainly in LAR, by controlling differentiation and migration of eosinophils.

MeSH terms

  • Administration, Inhalation
  • Airway Resistance / drug effects*
  • Animals
  • Antibodies / immunology
  • Asthma / immunology*
  • Bronchi / immunology
  • Bronchoalveolar Lavage Fluid / chemistry*
  • Cell Movement / drug effects
  • Cilia / drug effects
  • Cilia / pathology
  • Eosinophils / drug effects
  • Epithelial Cells / drug effects
  • Epithelial Cells / pathology
  • Epithelium / ultrastructure
  • Guinea Pigs
  • Intercellular Adhesion Molecule-1 / immunology
  • Intercellular Adhesion Molecule-1 / physiology*
  • Male
  • Microscopy, Electron
  • Microscopy, Electron, Scanning
  • Time Factors

Substances

  • Antibodies
  • Intercellular Adhesion Molecule-1