Abstract
We tested the role of group I mGluRs in the induction of long-term depression (LTD) in the visual cortex, using the novel mGluR antagonist LY341495 and mice lacking mGluR5, the predominant phosphoinositide (PI)-linked mGluR in the visual cortex. We find that LY341495 is a potent blocker of glutamate-stimulated PI hydrolysis in visual cortical synaptoneurosomes, and that it effectively antagonizes the actions of the mGluR agonist 1S, 3R-aminocyclopentane-1,3-dicarboxylic acid (ACPD) on synaptic transmission in visual cortical slices. However, LY341495 has no effect on the induction of LTD by low-frequency stimulation. Furthermore, mice lacking mGluR5 show normal NMDA receptor-dependent LTD. These results indicate that group I mGluR activation is not required for the induction of NMDA receptor-dependent LTD in the visual cortex.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acids / pharmacology
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Animals
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Cycloleucine / analogs & derivatives
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Cycloleucine / pharmacology
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Depression, Chemical
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Excitatory Amino Acid Antagonists / pharmacology
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Hydrolysis
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In Vitro Techniques
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Methoxyhydroxyphenylglycol / analogs & derivatives
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Methoxyhydroxyphenylglycol / pharmacology
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Mice
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Mice, Knockout
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Neuronal Plasticity / drug effects
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Neuronal Plasticity / physiology*
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Phosphatidylinositols / metabolism
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Rats
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Receptors, Metabotropic Glutamate / agonists
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Receptors, Metabotropic Glutamate / antagonists & inhibitors
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Receptors, Metabotropic Glutamate / physiology*
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Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors
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Receptors, N-Methyl-D-Aspartate / genetics
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Receptors, N-Methyl-D-Aspartate / physiology*
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Synaptosomes / drug effects
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Synaptosomes / metabolism
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Visual Cortex / drug effects
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Visual Cortex / physiology*
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Xanthenes / pharmacology
Substances
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Amino Acids
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Excitatory Amino Acid Antagonists
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LY 341495
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Phosphatidylinositols
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Receptors, Metabotropic Glutamate
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Receptors, N-Methyl-D-Aspartate
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Xanthenes
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Cycloleucine
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1-amino-1,3-dicarboxycyclopentane
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Methoxyhydroxyphenylglycol
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3,4-dihydroxyphenylglycol