Single-tube multiplex-PCR screen for common deletional determinants of alpha-thalassemia

Blood. 2000 Jan 1;95(1):360-2.

Abstract

Alpha-thalassemia is very common throughout all tropical and subtropical regions of the world. In Southeast Asia and the Mediterranean regions, compound heterozygotes and homozygotes may have anemia that is mild to severe (hemoglobin [Hb] H disease) or lethal (Hb Bart's hydrops fetalis). We have developed a reliable, single-tube multiplex-polymerase chain reaction (PCR) assay for the 6 most frequently observed determinants of alpha-thalassemia. The assay allows simple, high throughput genetic screening for these common hematological disorders. (Blood. 2000;95:360-362)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA / blood*
  • DNA Primers
  • Genetic Testing / methods*
  • Globins / genetics*
  • Heterozygote
  • Homozygote
  • Humans
  • Multigene Family
  • Polymerase Chain Reaction / methods*
  • Sequence Deletion*
  • alpha-Thalassemia / genetics*

Substances

  • DNA Primers
  • Globins
  • DNA