Abstract
Using a variety of techniques, including limiting dilution assays (LDA), intracellular IFNgamma assays, and Db-IgG1 MHC dimer staining to measure viral peptide-specific T cell number and function, we show here that heterologous virus infections quantitatively delete and qualitatively alter the memory pool of T cells specific to a previously encountered virus. We also show that a prior history of a virus infection can alter the hierarchy of the immunodominant peptide response to a second virus and that virus infections selectively reactivate memory T cells with distinct specificities to earlier viruses. These results are consistent with a model for the immune system that accommodates memory T cell populations for multiple pathogens over the course of a lifetime.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, Viral / immunology
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CD8-Positive T-Lymphocytes / immunology*
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Cell Line, Transformed
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Dimerization
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Epitopes, T-Lymphocyte / immunology*
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H-2 Antigens / immunology
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Histocompatibility Antigen H-2D
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Immunodominant Epitopes / immunology*
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Immunoglobulin G / immunology
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Immunologic Memory / immunology*
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Interferon-gamma / analysis
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Lymphocytic choriomeningitis virus / immunology
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Major Histocompatibility Complex / immunology
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Muromegalovirus / immunology
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Pichinde virus / immunology
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Receptors, Antigen, T-Cell, alpha-beta / immunology
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Staining and Labeling
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Vaccinia virus / immunology
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Virus Diseases / immunology*
Substances
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Antigens, Viral
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Epitopes, T-Lymphocyte
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H-2 Antigens
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Histocompatibility Antigen H-2D
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Immunodominant Epitopes
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Immunoglobulin G
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Receptors, Antigen, T-Cell, alpha-beta
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T cell receptor peptide Vbeta8.1
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Interferon-gamma