Abstract
The correct site for translation initiation for Escherichia coli WecA (Rfe), presumably involved in catalyzing the transfer of N-acetylglucosamine 1-phosphate to undecaprenylphosphate, was determined by using its FLAG-tagged derivatives. The N-terminal region containing three predicted transmembrane helices was found to be necessary for function but not for membrane localization of this protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylglucosamine / analogs & derivatives
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Acetylglucosamine / metabolism
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Amino Acid Sequence
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Bacterial Proteins / chemistry*
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Base Sequence
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Catalysis
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Cell Membrane / metabolism
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Escherichia coli
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Hexosyltransferases*
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Molecular Sequence Data
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Oligopeptides
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Peptides / metabolism
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Protein Biosynthesis*
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Protein Structure, Secondary*
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Structure-Activity Relationship
Substances
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Bacterial Proteins
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Oligopeptides
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Peptides
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N-acetylglucosamine-1-phosphate
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FLAG peptide
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Hexosyltransferases
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RgpG protein, Streptococcus mutans
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Acetylglucosamine