Abstract
A gamma-carboxyglutamic acid-containing protein has been purified from the calcified tissues of several vertebrates. The presence of three-gamma-carboxyglutamic acid residues in the bovine protein was established by alkaline hydrolysis and amino acid analysis, a method based upon studies with synthetic gamma-carboxyglutamic acid. The identity of gamma-carboxyglutamic acid in the bovine protein was established by mass spectroscopy on the unknown amino acid isolated from alkaline hydrolysates.
MeSH terms
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Age Factors
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Amino Acid Sequence
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Animals
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Calcification, Physiologic / drug effects
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Cattle
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Dentin / analysis
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Depression, Chemical
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Female
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Femur / analysis*
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Fishes
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Humans
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Hydroxyapatites / metabolism
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Lipoproteins / biosynthesis*
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Male
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Proteins / analysis*
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Proteins / isolation & purification
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Proteins / metabolism
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Proteins / pharmacology
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Tricarboxylic Acids
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Vitellogenins / biosynthesis*
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Vitellogenins / blood
Substances
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Hydroxyapatites
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Lipoproteins
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Proteins
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Tricarboxylic Acids
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Vitellogenins