Abstract
We have investigated noninvasive immunization to plague. Recombinant subunit antigens, F1 and V from Yersinia pestis, were coencapsulated in biodegradable poly(L100 LD(50's) inhalational challenge with virulent Y. pestis. These data expand on previous findings from our laboratories, providing further insight into the mechanics of safeguarding mice from plague through nasal immunization. Further, these results demonstrate that in a murine model, solid protection from pneumonic plague can be engendered by two intranasal administrations of appropriately formulated recombinant proteins.
MeSH terms
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Administration, Intranasal
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Animals
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Antibodies, Bacterial / blood
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Antigens, Bacterial / administration & dosage*
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Antigens, Bacterial / genetics
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Antigens, Bacterial / immunology
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Bacterial Proteins / administration & dosage*
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Bacterial Proteins / genetics
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Bacterial Proteins / immunology
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Bacterial Vaccines / administration & dosage*
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Bacterial Vaccines / genetics
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Bacterial Vaccines / immunology
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Biocompatible Materials / administration & dosage
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Biocompatible Materials / chemistry
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Dose-Response Relationship, Immunologic
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Female
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Immunity, Cellular / immunology
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Immunoglobulin G / blood
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Mice
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Mice, Inbred BALB C
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Microspheres
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Nasal Mucosa / immunology*
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Plague / immunology*
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Plague / prevention & control*
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Polyesters / administration & dosage
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Polyesters / chemistry
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Pore Forming Cytotoxic Proteins
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Vaccines, Synthetic / administration & dosage*
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Vaccines, Synthetic / immunology
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Yersinia pestis / genetics
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Yersinia pestis / immunology
Substances
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Antibodies, Bacterial
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Antigens, Bacterial
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Bacterial Proteins
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Bacterial Vaccines
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Biocompatible Materials
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Immunoglobulin G
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LcrV protein, Yersinia
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Polyesters
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Pore Forming Cytotoxic Proteins
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Vaccines, Synthetic
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caf1 protein, Yersinia pestis
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poly(lactide)