Agglutinating activity of alcohol-soluble proteins from quinoa seed flour in celiac disease

Plant Foods Hum Nutr. 1999;54(2):93-100. doi: 10.1023/a:1008059519025.

Abstract

The edible seeds of the quinoa plant contain small quantities of alcohol-soluble protein which, after peptic-tryptic digestion, are unable to agglutinate K562(s) cells. When separated by affinity chromatography on sepharose-6B coupled with mannan, peptic-tryptic digest separated in two fractions. Fraction B peptides (about 1% of total protein) were shown to agglutinate K562(s) cells at a very low concentration, whereas peptides in fraction A and in the mixed fraction A+B were inactive, suggesting that fraction A contains protective peptides that interfere with the agglutinating activity of toxic peptides in fraction B.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agglutination / drug effects*
  • Amino Acids / analysis
  • Celiac Disease / metabolism*
  • Chromatography, Affinity
  • Enzyme-Linked Immunosorbent Assay
  • Flour
  • Plant Proteins / analysis
  • Plant Proteins / isolation & purification
  • Plant Proteins / pharmacology*
  • Seeds / chemistry*
  • Solubility

Substances

  • Amino Acids
  • Plant Proteins