Cloning and sequencing of an alkaline protease gene from Bacillus lentus and amplification of the gene on the B. lentus chromosome by an improved technique

P L Jørgensen; M Tangney; P E Pedersen; S Hastrup; B Diderichsen; S T Jørgensen

doi:10.1128/AEM.66.2.825-827.2000

Cloning and sequencing of an alkaline protease gene from Bacillus lentus and amplification of the gene on the B. lentus chromosome by an improved technique

Appl Environ Microbiol. 2000 Feb;66(2):825-7. doi: 10.1128/AEM.66.2.825-827.2000.

Authors

P L Jørgensen¹, M Tangney, P E Pedersen, S Hastrup, B Diderichsen, S T Jørgensen

Affiliation

¹ Bacterial Gene Technology, Novo Nordisk A/S, 2880 Bagsvaerd, Copenhagen, Denmark.

Abstract

A gene encoding an alkaline protease was cloned from an alkalophilic bacillus, and its nucleotide sequence was determined. The cloned gene was used to increase the copy number of the protease gene on the chromosome by an improved gene amplification technique.

MeSH terms

Bacillus / enzymology*
Bacillus / genetics
Cloning, Molecular*
Endopeptidases / genetics*
Endopeptidases / metabolism*
Hydrogen-Ion Concentration
Molecular Sequence Data
Nucleic Acid Amplification Techniques*
Plasmids / genetics
Sequence Analysis, DNA

Substances

Endopeptidases

Associated data

SWISSPROT/P29600