Abstract
CD27, a member of the TNF receptor superfamily, has been implicated in T cell activation, T cell development, and T cell-dependent Ab production by B cells. In the present study we examined the expression and function of CD27 on murine NK cells. Murine NK cells constitutively expressed CD27 on their surface. Stimulation with immobilized anti-CD27 mAb or murine CD27 ligand (CD70) transfectans solely could induce proliferation and IFN-gamma production of freshly isolated NK cells and enhanced the proliferation and IFN-gamma production of anti-NK1.1-sutimulated NK cells. Although NK cell cytotoxicity was not triggered by anti-CD27 mAb or against CD70 transfectants, prestimulation via CD27 enhanced the cytotoxic activity of NK cells in an IFN-gamma-dependent manner. These results suggest that CD27-mediated activation may be involved in the NK cell-mediated innate immunity against virus-infected or transformed cells expressing CD70.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / physiology
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Animals
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Antibodies, Monoclonal / metabolism
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Antigens, CD*
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CD27 Ligand
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Cells, Cultured
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Cytotoxicity, Immunologic / immunology
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Interferon-gamma / biosynthesis
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Interferon-gamma / physiology
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Killer Cells, Natural / immunology*
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Killer Cells, Natural / metabolism
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Ligands
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Lymphocyte Activation / immunology*
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Male
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Membrane Proteins / physiology
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Mice
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Mice, Inbred C57BL
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Tumor Cells, Cultured
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Tumor Necrosis Factor Receptor Superfamily, Member 7 / biosynthesis
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Tumor Necrosis Factor Receptor Superfamily, Member 7 / immunology
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Tumor Necrosis Factor Receptor Superfamily, Member 7 / metabolism
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Tumor Necrosis Factor Receptor Superfamily, Member 7 / physiology*
Substances
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Adjuvants, Immunologic
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Antibodies, Monoclonal
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Antigens, CD
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CD27 Ligand
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Cd70 protein, mouse
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Ligands
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Membrane Proteins
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Tumor Necrosis Factor Receptor Superfamily, Member 7
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Interferon-gamma