Proper alignment of a needle template and ultrasound software grid is required to accurately deliver permanent prostate seed implants optimized using pretreatment volume studies. Correct alignment may also reduce tissue edema, morbidity, and the time and labor required to deliver permanent prostate seed implants. A technique has been developed to rapidly assess (and, if necessary, improve) the alignment using a custom-designed water phantom. Verification of needle positions can be conducted within 1 mm and requires less than ten minutes. We have instituted the technique as a part of our periodic quality assurance program.