Abstract
We have identified the 30-zinc finger protein OAZ as a DNA-binding factor that associates with Smads in response to BMP2, forming a complex that transcriptionally activates the homeobox regulator of Xenopus mesoderm and neural development, Xvent-2. OAZ contains a BMP signaling module formed by two clusters of fingers that bind Smads and the Xvent-2 BMP response element, respectively. Previously implicated as a transcriptional partner of Olf-1/EBF in olfactory epithelium and lymphocyte development in the rat, OAZ fulfills this role through clusters of fingers that are separate from the BMP signaling module. The mutually exclusive use of OAZ by the BMP-Smad and Olf pathways illustrates the dual role of a multi-zinc finger protein in signal transduction during development.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
Basic Helix-Loop-Helix Transcription Factors
-
Bone Morphogenetic Proteins / genetics*
-
Bone Morphogenetic Proteins / metabolism
-
COS Cells
-
Cell Nucleus / chemistry
-
Cloning, Molecular
-
DNA-Binding Proteins / analysis
-
DNA-Binding Proteins / genetics*
-
DNA-Binding Proteins / metabolism
-
DNA-Binding Proteins / physiology*
-
Gene Expression Regulation, Developmental / physiology
-
Genes, Reporter
-
Homeodomain Proteins / genetics
-
Homeodomain Proteins / metabolism
-
Humans
-
Lac Operon
-
Molecular Sequence Data
-
Nerve Tissue Proteins / metabolism
-
Oocytes / physiology
-
Protein Binding / physiology
-
Proteins
-
Rats
-
Signal Transduction / physiology*
-
Smad Proteins
-
Smad2 Protein
-
Smad3 Protein
-
Trans-Activators / physiology*
-
Transcription Factors / analysis
-
Transcription Factors / genetics*
-
Transcription Factors / metabolism
-
Transcription Factors / physiology*
-
Transcription, Genetic / physiology
-
Xenopus
-
Xenopus Proteins / analysis
-
Xenopus Proteins / genetics*
-
Xenopus Proteins / metabolism
-
Xenopus Proteins / physiology*
-
Yeasts / genetics
-
Zinc Fingers / genetics*
Substances
-
BABAM2 protein, human
-
Basic Helix-Loop-Helix Transcription Factors
-
Bone Morphogenetic Proteins
-
DNA-Binding Proteins
-
EBF1 protein, human
-
Ebf1 protein, rat
-
Homeodomain Proteins
-
Nerve Tissue Proteins
-
Proteins
-
SMAD2 protein, human
-
SMAD3 protein, human
-
Smad Proteins
-
Smad2 Protein
-
Smad2 protein, Xenopus
-
Smad2 protein, rat
-
Smad3 Protein
-
Smad3 protein, rat
-
Trans-Activators
-
Transcription Factors
-
Xenopus Proteins
-
ZNF423 protein, Xenopus
-
ebf2 protein, Xenopus
-
madh3 protein, Xenopus
-
ornithine decarboxylase antizyme
-
ventx1.2 protein, Xenopus
-
ventx2.1 protein, Xenopus