Fas-resistance in ATL cell lines not associated with HTLV-I or FAP-1 production

Y Yamada; K Sugahara; K Tsuruda; K Nohda; T Hata; T Maeda; M Honda; M Tawara; T Hayashibara; T Joh; M Tomonaga; Y Miyazaki; S Kamihira

doi:10.1016/s0304-3835(99)00313-4

Fas-resistance in ATL cell lines not associated with HTLV-I or FAP-1 production

Cancer Lett. 1999 Dec 1;147(1-2):215-9. doi: 10.1016/s0304-3835(99)00313-4.

Authors

Y Yamada¹, K Sugahara, K Tsuruda, K Nohda, T Hata, T Maeda, M Honda, M Tawara, T Hayashibara, T Joh, M Tomonaga, Y Miyazaki, S Kamihira

Affiliation

¹ Department of Laboratory Medicine, Nagasaki University School of Medicine, Japan.

PMID: 10660109
DOI: 10.1016/s0304-3835(99)00313-4

Abstract

A preventive role for human T-cell leukemia virus type-I (HTLV-I) and Fas-associated phosphatase-1 (FAP-1) in Fas-mediated apoptosis has been reported in HTLV-I-infected cells. In the present study, we examined whether these molecules increased during the acquisition of Fas-resistance in adult T-cell leukemia (ATL) cell lines. SO4, ST1 and KK1 are Fas-sensitive ATL cell lines, and produce small amounts of HTLV-I in vitro. Although their subclones RSO4 and RST1 are completely Fas-resistant, they produced an equivalent amount of HTLV-I to SO4 and ST1. Moreover, FAP-1 mRNA was not detected in these cell lines irrespective of Fas sensitivity. Thus, Fas resistance in ATL cells was not directly associated with the increased production of HTLV-I or FAP-1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal / pharmacology
Apoptosis / drug effects
Apoptosis / immunology*
Blotting, Southern
Carrier Proteins / biosynthesis*
Carrier Proteins / genetics
Carrier Proteins / immunology
Clone Cells
DNA Fragmentation / drug effects
DNA, Complementary / biosynthesis
Drug Resistance, Neoplasm
Human T-lymphotropic virus 1 / genetics
Human T-lymphotropic virus 1 / isolation & purification*
Humans
Leukemia, T-Cell / genetics
Leukemia, T-Cell / metabolism*
Leukemia, T-Cell / pathology
Leukemia, T-Cell / virology*
Protein Phosphatase 1
Protein Tyrosine Phosphatase, Non-Receptor Type 13
Protein Tyrosine Phosphatases / biosynthesis*
Protein Tyrosine Phosphatases / genetics
Protein Tyrosine Phosphatases / immunology
Proto-Oncogene Proteins c-bcl-2 / biosynthesis
RNA, Messenger / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Virus Integration / genetics
fas Receptor / immunology*
fas Receptor / pharmacology

Substances

Antibodies, Monoclonal
Carrier Proteins
DNA, Complementary
Proto-Oncogene Proteins c-bcl-2
RNA, Messenger
fas Receptor
Protein Phosphatase 1
PTPN13 protein, human
Protein Tyrosine Phosphatase, Non-Receptor Type 13
Protein Tyrosine Phosphatases