Crystallization and preliminary X-ray analysis of the thymidylate kinase from Mycobacterium tuberculosis

I Li de la Sierra; H Munier-Lehmann; A M Gilles; O Bârzu; M Delarue

doi:10.1107/s0907444999016212

Crystallization and preliminary X-ray analysis of the thymidylate kinase from Mycobacterium tuberculosis

Acta Crystallogr D Biol Crystallogr. 2000 Feb;56(Pt 2):226-8. doi: 10.1107/s0907444999016212.

Authors

I Li de la Sierra¹, H Munier-Lehmann, A M Gilles, O Bârzu, M Delarue

Affiliation

¹ Unité de Biochimie Structurale, Institut Pasteur, 28 Rue du Dr Roux, 75724, Paris CEDEX 15, France.

PMID: 10666613
DOI: 10.1107/s0907444999016212

Abstract

Mycobacterium tuberculosis thymidylate kinase complexed with the substrate deoxythymidine monophosphate was crystallized in the hexagonal space group P6(5)22 or P6(1)22, with unit-cell parameters a = b = 76.62, c = 134.38 A and one single monomer of 23 kDa in the asymmetric unit. Cryo-cooled crystals diffract at 1.94 A resolution using synchrotron radiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / chemistry
Bacterial Proteins / metabolism
Crystallization
Mycobacterium tuberculosis / enzymology*
Nucleoside-Phosphate Kinase / chemistry*
Nucleoside-Phosphate Kinase / metabolism
Thymidine Monophosphate / chemistry
Thymidine Monophosphate / metabolism
X-Ray Diffraction

Substances

Bacterial Proteins
Thymidine Monophosphate
Nucleoside-Phosphate Kinase
dTMP kinase