Capillary electrophoretic determination of sanguinarine and chelerythrine in plant extracts and pharmaceutical preparations

J Chromatogr A. 2000 Jan 14;866(2):293-8. doi: 10.1016/s0021-9673(99)01126-7.

Abstract

Capillary electrophoresis was employed to determine the principal quaternary benzo[c]phenanthridine alkaloids, sanguinarine and chelerythrine, in two plant extracts and one oral hygiene product. Phosphate-Tris buffer of pH 2.5 was used as a background electrolyte, limits of detection were 3 micromol/l(-1) (sanguinarine) and 2.4 micromol,l(-1) (chelerythrine) using UV detection at 270 nm. The method, which correlated well with HPLC, is suitable for serial determination of sanguinarine and chelerythrine in plant products and pharmaceuticals.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids / analysis*
  • Benzophenanthridines
  • Chromatography, High Pressure Liquid
  • Electrophoresis, Capillary / methods*
  • Isoquinolines
  • Pharmaceutical Preparations / chemistry*
  • Phenanthridines / analysis*
  • Plant Extracts / chemistry*
  • Sensitivity and Specificity

Substances

  • Alkaloids
  • Benzophenanthridines
  • Isoquinolines
  • Pharmaceutical Preparations
  • Phenanthridines
  • Plant Extracts
  • sanguinarine
  • chelerythrine