Determination of sugar conformation in large RNA oligonucleotides from analysis of dipole-dipole cross correlated relaxation by solution NMR spectroscopy

C Richter; C Griesinger; I Felli; P T Cole; G Varani; H Schwalbe

doi:10.1023/a:1008319130714

Determination of sugar conformation in large RNA oligonucleotides from analysis of dipole-dipole cross correlated relaxation by solution NMR spectroscopy

J Biomol NMR. 1999 Nov;15(3):241-50. doi: 10.1023/a:1008319130714.

Authors

C Richter¹, C Griesinger, I Felli, P T Cole, G Varani, H Schwalbe

Affiliation

¹ Institut für Organische Chemie, Johann-Wolfgang-Goethe-Universität Frankfurt, Germany.

PMID: 10677827
DOI: 10.1023/a:1008319130714

Abstract

A new experiment, the forward directed quantitative gamma-HCCH-TOCSY for the measurement of the conformation of the five-membered ribosyl unit in RNA oligonucleotides, is presented. The experiment relies on quantification of cross peak intensities caused by evolution of CH, CH-dipole-dipole cross correlated relaxation in non-evolution periods and the resolution enhancement obtainable in forward directed HCC-TOCSY transfer. Cross correlated relaxation rates are interpreted to reveal the sugar conformation of 22 out of 25 nucleotides in an isotopically labelled 25-mer RNA. The results obtained with this new method are in agreement with the conformational analysis derived from 3J(H,H) coupling constants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Conformation
Models, Theoretical
Nuclear Magnetic Resonance, Biomolecular / instrumentation
Nuclear Magnetic Resonance, Biomolecular / methods*
Nucleic Acid Conformation
Oligoribonucleotides / chemistry*
Solutions

Substances

Oligoribonucleotides
Solutions