Time-dependent effect of cyclosporine on mitogenic responses and lymphocyte subset populations in rat spleen and submaxillary lymph nodes

Neuroimmunomodulation. 2000;7(2):84-8. doi: 10.1159/000026424.

Abstract

Lipopolysaccharide (LPS)- and concanavalin A (ConA)-induced proliferation and T lymphocyte subsets were measured in spleen and submaxillary lymph nodes of male rats injected with cyclosporine (5 mg/kg s.c. for 5 days, at 12.00 or 24.00 h; animals kept under light from 08.00 to 20.00 h daily). One hour before the third injection, Freund's complete adjuvant or its vehicle was administered. A suppressive effect of cyclosporine on the mitogenic action of LPS was seen in the spleen of rats injected with cyclosporine at noon whereas the effect of ConA remained unaffected. CD4+, CD8+ and CD4+-CD8+ cells decreased in spleen and lymph nodes of Freund's adjuvant-injected rats only with cyclosporine given at noon. The results further support occurrence of time-of-day-dependent effects of cyclosporine on lymphocyte subsets and proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Division / drug effects
  • Chronobiology Phenomena*
  • Concanavalin A / antagonists & inhibitors
  • Concanavalin A / pharmacology
  • Cyclosporine / administration & dosage
  • Cyclosporine / pharmacology*
  • Freund's Adjuvant / administration & dosage
  • Freund's Adjuvant / immunology
  • Immunosuppressive Agents / administration & dosage
  • Immunosuppressive Agents / pharmacology*
  • Lipopolysaccharides / antagonists & inhibitors
  • Lipopolysaccharides / pharmacology
  • Lymph Nodes / drug effects
  • Lymph Nodes / immunology
  • Lymphocyte Activation / drug effects*
  • Lymphocyte Subsets / cytology
  • Lymphocyte Subsets / drug effects*
  • Lymphocyte Subsets / immunology
  • Male
  • Mitogens / antagonists & inhibitors
  • Mitogens / pharmacology*
  • Rats
  • Rats, Wistar
  • Spleen / drug effects
  • Spleen / immunology
  • Submandibular Gland / drug effects
  • Submandibular Gland / immunology
  • T-Lymphocytes / cytology
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology

Substances

  • Immunosuppressive Agents
  • Lipopolysaccharides
  • Mitogens
  • Concanavalin A
  • Cyclosporine
  • Freund's Adjuvant