Modelisation of leukocyte adhesion on a fibrinogen coated surface in static conditions

Clin Hemorheol Microcirc. 1999;21(3-4):375-82.

Abstract

The adhesion of polymorphonuclear leukocytes (PMNs) on the vascular endothelium is a complex process that occurs during biological and pathological events and involves a large family of molecules. This phenomenom could be approached by a modelisation study of the adhesion of PMNs on a biological substrate, fibrinogen. Two different physiological conditions were tested such as the activated state of PMNs with a synthetic pro-inflammatory activator (N-Formyl-Methionyl-Leucyl-Phenylalanine, FMLP). The activated state of PMNs was both quantified by flow cytometry and controlled by fluorescence microscopy. The results suggest that quiescent PMNs deposit in accordance with the ballistic deposition model. The preliminary results obtained with FMLP-stimulated PMNs show a different deposit process compared to quiescent PMNs but do not allow to determine exactly a deposition model.

MeSH terms

  • Activated-Leukocyte Cell Adhesion Molecule / analysis
  • Cell Adhesion / drug effects
  • Cell Adhesion / physiology
  • Fibrinogen / metabolism*
  • Fibrinogen / pharmacology
  • Flow Cytometry
  • Humans
  • Leukocyte Count / drug effects
  • Leukocytes / drug effects
  • Leukocytes / physiology*
  • Microscopy, Fluorescence
  • Models, Biological*
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Surface Properties / drug effects

Substances

  • Activated-Leukocyte Cell Adhesion Molecule
  • N-Formylmethionine Leucyl-Phenylalanine
  • Fibrinogen