Interleukin-1beta induction of the chemokine RANTES promoter in the human astrocytoma line CH235 requires both constitutive and inducible transcription factors

J Neuroimmunol. 2000 Jun 1;105(1):78-90. doi: 10.1016/s0165-5728(00)00195-8.

Abstract

The chemokine RANTES is an important mediator of inflammatory processes. In this report, we describe the DNA sequence and transcription factor requirements for interleukin-1beta (IL-1beta) induction of the RANTES promoter in the human astrocytoma line CH235. RANTES promoter sequences between -278 and +55 are sufficient for IL-1beta-inducibility. In vitro DNA binding assays demonstrate constitutive binding of Sp1, HMG, Ets domain, and bZIP family members to their cognate sites in the RANTES promoter, whereas NF-kappaB and IRF-1 bind in an IL-1beta-inducible manner. IL-1beta-inducibility of the RANTES promoter requires both constitutive and inducible transcription factors. The formation of a higher order nucleoprotein complex, or 'enhanceosome', may be critical for IL-1beta induction of the RANTES promoter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Astrocytoma / metabolism*
  • Astrocytoma / pathology
  • Basic-Leucine Zipper Transcription Factors
  • Binding Sites
  • Chemokine CCL5 / genetics*
  • DNA-Binding Proteins / metabolism
  • G-Box Binding Factors
  • Gene Expression Regulation / drug effects*
  • Humans
  • Interleukin-1 / pharmacology*
  • NF-kappa B / metabolism
  • Promoter Regions, Genetic*
  • Sp1 Transcription Factor / metabolism
  • Transcription Factors / metabolism
  • Transcription Factors / physiology*
  • Tumor Cells, Cultured

Substances

  • Basic-Leucine Zipper Transcription Factors
  • Chemokine CCL5
  • DNA-Binding Proteins
  • G-Box Binding Factors
  • Interleukin-1
  • NF-kappa B
  • Sp1 Transcription Factor
  • Transcription Factors