A novel experiment for the quantitative measurement of CSA(1H(N))/CSA(15N) cross-correlated relaxation in 15N-labeled proteins

J Biomol NMR. 2000 Feb;16(2):171-4. doi: 10.1023/a:1008347012429.

Abstract

An experiment is presented which allows for the quantitative measurement of the relaxation interference between the 1H(N) CSA and 15N CSA interactions in 15N labeled proteins. A constant-time buildup scheme is used to measure the differential relaxation rate, eta, between double-quantum (DQ) and zero-quantum (ZQ) 1H(N)-15N coherences. The CSA/CSA experiment was recorded at three different Bo field strengths. The CSA(1H(N))/CSA(15N) cross-correlation rate was obtained from the linear fit of the measured rate, eta, versus Bo2 for 77 residues of the EH2 domain from mouse Eps15.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acids / chemistry
  • Animals
  • Calcium-Binding Proteins / chemistry
  • Intracellular Signaling Peptides and Proteins
  • Mice
  • Models, Chemical
  • Nitrogen Isotopes
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Phosphoproteins / chemistry
  • Proteins / chemistry*
  • Reference Standards

Substances

  • Adaptor Proteins, Signal Transducing
  • Amino Acids
  • Calcium-Binding Proteins
  • Eps15 protein, mouse
  • Intracellular Signaling Peptides and Proteins
  • Nitrogen Isotopes
  • Phosphoproteins
  • Proteins