A Drosophila complementary DNA resource

G M Rubin; L Hong; P Brokstein; M Evans-Holm; E Frise; M Stapleton; D A Harvey

doi:10.1126/science.287.5461.2222

A Drosophila complementary DNA resource

Science. 2000 Mar 24;287(5461):2222-4. doi: 10.1126/science.287.5461.2222.

Authors

G M Rubin¹, L Hong, P Brokstein, M Evans-Holm, E Frise, M Stapleton, D A Harvey

Affiliation

¹ Berkeley Drosophila Genome Project, Howard Hughes Medical Institute, and Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA.

PMID: 10731138
DOI: 10.1126/science.287.5461.2222

Abstract

Collections of nonredundant, full-length complementary DNA (cDNA) clones for each of the model organisms and humans will be important resources for studies of gene structure and function. We describe a general strategy for producing such collections and its implementation, which so far has generated a set of cDNAs corresponding to over 40% of the genes in the fruit fly Drosophila melanogaster.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

3' Untranslated Regions
5' Untranslated Regions
Animals
Cloning, Molecular
DNA, Complementary*
Drosophila melanogaster / genetics*
Expressed Sequence Tags
Gene Library*
Genes, Insect*
Open Reading Frames

Substances

3' Untranslated Regions
5' Untranslated Regions
DNA, Complementary