Abstract
Recombinant plasmid DNA constructs expressing truncated or full-length dengue-1 envelope (E) with or without the pre-membrane (prM) were tested for immunogenicity in mice, as candidate dengue DNA vaccines. Two plasmids, one expressing the N-terminal 80% E and the other expressing prM and full length E were immunogenic in intradermally inoculated mice. The vaccinated mice produced dengue-1 specific antibodies that were both neutralizing and long lasting. Data suggested that the plasmid expressing prM and full length E produced virus like particles in transfected cells, and is probably a better immunogen compared to that expressing 80% E.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Antibodies, Viral / biosynthesis
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Base Sequence
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Cell Line
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DNA Primers / genetics
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Dengue / immunology
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Dengue / prevention & control
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Dengue Virus / genetics
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Dengue Virus / immunology*
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Humans
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Immunization
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Mice
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Mice, Inbred BALB C
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Neutralization Tests
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Peptide Fragments / genetics
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Peptide Fragments / immunology
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Vaccines, DNA / genetics
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Vaccines, DNA / immunology*
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology*
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Viral Vaccines / genetics
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Viral Vaccines / immunology*
Substances
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Antibodies, Viral
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DNA Primers
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Peptide Fragments
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Recombinant Proteins
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Vaccines, DNA
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Vaccines, Synthetic
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Viral Envelope Proteins
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Viral Vaccines