Immune responses induced by BCG recombinant for human papillomavirus L1 and E7 proteins

I A Jabbar; G J Fernando; N Saunders; A Aldovini; R Young; K Malcolm; I H Frazer

doi:10.1016/s0264-410x(99)00550-2

Immune responses induced by BCG recombinant for human papillomavirus L1 and E7 proteins

Vaccine. 2000 May 8;18(22):2444-53. doi: 10.1016/s0264-410x(99)00550-2.

Authors

I A Jabbar¹, G J Fernando, N Saunders, A Aldovini, R Young, K Malcolm, I H Frazer

Affiliation

¹ Centre for Immunology and Cancer Research, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.

PMID: 10738102
DOI: 10.1016/s0264-410x(99)00550-2

Abstract

Recombinant bacille Calmette-Guerin (BCG) based vaccine delivery systems could potentially share the safety and effectiveness of BCG. We therefore prepared recombinant BCG vaccines which expressed the L1 late protein of the human papillomavirus (HPV) 6b or the E7 early protein of the HPV 16. The two recombinants were evaluated as immunogens in C57BL/6J and BALB/c mice, and compared with a conventional protein/adjuvant system using E7 or L1 mixed with Quil-A adjuvant. rBCG6bL1 and rBCG16E7 primed specific immune responses, represented by DTH, T-proliferation and antibody, and rBCG16E7 induced cytotoxic immune response to E7 protein. The magnitude of the observed responses were less than those elicited by protein/adjuvant vaccine. As recombinant BCG vaccines expressing HPV6bL1 or HPV16E7 persist at low levels in the immunised host, they may be beneficial to prime or retain memory responses to antigens, but are unlikely to be useful as a single component vaccine strategy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic / administration & dosage
Animals
Antibodies, Viral / biosynthesis
BCG Vaccine / administration & dosage
BCG Vaccine / genetics
BCG Vaccine / immunology*
Base Sequence
Capsid Proteins*
Cloning, Molecular
DNA Primers / genetics
Humans
Hypersensitivity, Delayed
Lymphocyte Activation
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Oncogene Proteins, Viral / genetics
Oncogene Proteins, Viral / immunology*
Papillomaviridae / genetics
Papillomaviridae / immunology*
Papillomavirus E7 Proteins
Plasmids / genetics
T-Lymphocytes / immunology
T-Lymphocytes, Cytotoxic / immunology
Vaccines, Synthetic / administration & dosage
Vaccines, Synthetic / genetics
Vaccines, Synthetic / immunology*
Viral Proteins

Substances

Adjuvants, Immunologic
Antibodies, Viral
BCG Vaccine
Capsid Proteins
DNA Primers
Oncogene Proteins, Viral
Papillomavirus E7 Proteins
Vaccines, Synthetic
Viral Proteins
oncogene protein E7, Human papillomavirus type 16
L1 protein, Human papillomavirus type 6