Objective: To locate lost region of tumor suppressor gene on chromosome 13q in squamous-cell carcinoma of the larynx (LSCC) and to provide clues and evidence for discovering and locating new suppressor gene.
Methods: Loss of heterozygosity (LOH) on chromosomes 13 was analyzed in 58 LSCC patients. By microsatellite polymorphic sequences in loci D13S765 (13q13), RB 1.20 (13q14.2), D13s133 (13q14.3) and D13S318 (13q21) on chromosome 13 by PCR.
Results: There weren't any LOH on chromosome 13q in 3 cases with preinvasive LSCC. Forty-five percentage (24/53) of the 53 invasive LSCC cases showed LOH at one or more loci on chromosome 13q region. The highest perentage of LOH on chromosome 13q was 52% (22/53) at D13S765 locus.
Conclusion: The deletion region on chromosome 13q was located near by D13S765 locus which is centrimeric to RB1. In this region there is suppressor gene, which related to the genesis and developement of LSCC, possibly including RB1. The inactivation of these suppressor gene may be related to the genesis and developement of invasive LSCC.