Objective: To evaluate DNA/Cytokeratin(CK) multiparametric method in detection to the gastric cancer and to study the relations of cellular DNA content, S-phase fraction (SPF) and G2/M-phase fraction (G2/MPF) to lymph node (LN) metastasis.
Methods: 50 cases of fresh tumor samples, were examined by using single (DNA) and two-colored DNA/CK flow cytometry respectively.
Results: The rate of aneuploid, the mean and the range of SPF and G2/MPF were higher in the multiparametric method than that in single one. In aneuploid carcinoma, the incidence of LN metastasis was significantly higher, the G2/MPF values with LN metastasis were higher than those without metastasis; values were higher in metastasis to n2 or n3 than that to n1. The SPF or G2/MPF values correlated with the number of LN metastasis, being significantly higher in metastasis to 5 LN than to 1-4 LN.
Conclusion: Multiparametric DNA/CK cytometric analysis is better than single DNA one; correlations between cellular DNA content, SPF, G2/MPF and the extent of LN matastasis exist.