Abstract
VanD-type Enterococcus faecium BM4416 was constitutively resistant to vancomycin and to teicoplanin by synthesis of peptidoglycan precursors ending in D-alanyl-D-lactate. Like E. faecium BM4339, the only VanD-type strain described so far, BM4416 produced an impaired D-alanine:D-alanine ligase. Unlike for BM4339, which had a 5-bp insertion in ddl, inactivation of the gene in BM4416 was due to insertion of IS19.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / pharmacology*
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Base Sequence
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DNA, Bacterial / analysis
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Enterococcus faecium / drug effects
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Enterococcus faecium / enzymology*
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Enterococcus faecium / genetics
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Molecular Sequence Data
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Mutagenesis, Insertional
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Peptide Synthases / genetics*
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Peptide Synthases / metabolism
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Teicoplanin / pharmacology
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Vancomycin / pharmacology
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Vancomycin Resistance / genetics*
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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Teicoplanin
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Vancomycin
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Peptide Synthases
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D-alanylalanine synthetase