Abstract
TNFalpha (100 U/ml, 24 h) upregulated intercellular adhesion molecule 1 (ICAM1) expression and fluid phase endocytosis (FPE) of horseradish peroxidase on brain microvascular endothelial cell (BMEC) culture. The protein kinase C (PKC) inhibitor staurosporin (0. 5-10 nM) antagonized ICAM1 expression and FPE due to TNFalpha, whereas the protein kinase A inhibitor H89 (0.5-10 nM) did not. These findings indicate that a PKC-dependent mechanism may affect TNFalpha signalling on different barrier properties of BMECs.
MeSH terms
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Animals
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Blood-Brain Barrier / drug effects
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Blood-Brain Barrier / physiology
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Brain / blood supply
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Brain / drug effects*
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Brain / metabolism*
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Cells, Cultured
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Cerebral Arteries / cytology
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Cerebral Arteries / drug effects*
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Cerebral Arteries / metabolism*
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Endocytosis / drug effects*
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Endocytosis / physiology*
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Endothelium, Vascular / cytology
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Endothelium, Vascular / drug effects*
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Endothelium, Vascular / metabolism*
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Intercellular Adhesion Molecule-1 / drug effects*
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Intercellular Adhesion Molecule-1 / metabolism*
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Male
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Multiple Sclerosis, Relapsing-Remitting / etiology
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Multiple Sclerosis, Relapsing-Remitting / physiopathology
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Protein Kinase C / antagonists & inhibitors
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Protein Kinase C / drug effects*
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Protein Kinase C / metabolism*
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Rats
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Rats, Wistar
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Tumor Necrosis Factor-alpha / metabolism*
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Tumor Necrosis Factor-alpha / pharmacology*
Substances
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Tumor Necrosis Factor-alpha
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Intercellular Adhesion Molecule-1
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Protein Kinase C