Abstract
We examined the diversity of HIV-1 subtypes in 11 adults from Hungary, using the heteroduplex mobility assay (HMA) and DNA sequencing. HMA results showed that HIV-1 gp120 sequences from 10 patients were of subtype B, whereas 1 patient, infected in Africa, carried a subtype C strain. DNA sequencing confirmed the HMA results and revealed a high intrasubtype diversity in the C2V3 region of env in different clade B isolates, which suggests multiple introduction of subtype B to Hungary. This study shows that subtype B is the predominant HIV-1 clade in Hungary.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acquired Immunodeficiency Syndrome / epidemiology
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Acquired Immunodeficiency Syndrome / virology
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Adult
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Amino Acid Sequence
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Consensus Sequence
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DNA, Viral / genetics*
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Female
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HIV Envelope Protein gp120 / genetics*
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HIV Infections / epidemiology*
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HIV Infections / virology
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HIV-1 / chemistry
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HIV-1 / genetics*
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Heteroduplex Analysis
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Homosexuality
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Humans
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Hungary / epidemiology
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Male
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Molecular Sequence Data
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Phylogeny
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Proviruses / genetics
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Sequence Alignment
Substances
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DNA, Viral
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HIV Envelope Protein gp120
Associated data
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GENBANK/AJ245461
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GENBANK/AJ245462
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GENBANK/AJ245463
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GENBANK/AJ245464
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GENBANK/AJ245465
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GENBANK/AJ245466
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GENBANK/AJ245467
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GENBANK/AJ245468
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GENBANK/AJ245469
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GENBANK/AJ245470
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GENBANK/AJ245471
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GENBANK/AJ245472