Detection of Chlamydia trachomatis-specific antibodies in human sera by recombinant major outer-membrane protein polyantigens

Per Mygind; Gunna Christiansen; Kenneth Persson; Svend Birkelund

doi:10.1099/0022-1317-49-5-457

Detection of Chlamydia trachomatis-specific antibodies in human sera by recombinant major outer-membrane protein polyantigens

J Med Microbiol. 2000 May;49(5):457-465. doi: 10.1099/0022-1317-49-5-457.

Authors

Per Mygind, Gunna Christiansen, Kenneth Persson¹, Svend Birkelund

Affiliation

¹ Department of Medical Microbiology and Immunology, University of Aarhus, DK-8000 Aarhus, Denmark and *Department of Virology, University Hospital, Malmö, Sweden.

PMID: 10798559
DOI: 10.1099/0022-1317-49-5-457

Abstract

This study was performed to generate and evaluate recombinant antigens for use in a species-specific Chlamydia trachomatis immunoassay. In a molecular genetic approach, fragments of the C. trachomatis major outer-membrane protein (MOMP) were produced as fusion proteins to create three different constructs encompassing the variable domains I, II and IV of selected C. trachomatis serovars. The recombinant MOMP polyantigens were affinity-purified and used in an enzyme-linked immunosorbent assay. Antibody detection was evaluated with 103 patient sera and the results were compared with titres obtained in the micro-immunofluorescence test. The results showed that the generated MOMP polyantigens detected the presence of C. trachomatis-specific human antibodies with little cross-reaction to C. pneumoniae-specific antibodies. When compared to the micro-immunofluorescence assay the MOMP polyantigen detected the presence of anti-C. trachomatis IgG antibodies with a sensitivity of 80% and a specificity of 91%.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Antibodies, Bacterial / blood*
Antigens, Bacterial / chemistry
Antigens, Bacterial / genetics
Antigens, Bacterial / immunology*
Antigens, Surface / chemistry
Antigens, Surface / genetics
Antigens, Surface / immunology
Bacterial Outer Membrane Proteins / chemistry
Bacterial Outer Membrane Proteins / genetics
Bacterial Outer Membrane Proteins / immunology
Chlamydia Infections / diagnosis*
Chlamydia trachomatis / genetics
Chlamydia trachomatis / immunology*
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
Female Urogenital Diseases / diagnosis*
Fluorescent Antibody Technique
Humans
Immunoglobulin G / blood
Male Urogenital Diseases*
Molecular Sequence Data
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / genetics
Sensitivity and Specificity
Sequence Alignment
Species Specificity

Substances

Antibodies, Bacterial
Antigens, Bacterial
Antigens, Surface
Bacterial Outer Membrane Proteins
Immunoglobulin G
Recombinant Fusion Proteins