[Preservation of erythrocytes of heterozygous SC sickle cell patients]

Transfus Clin Biol. 2000 Apr;7(2):119-28. doi: 10.1016/s1246-7820(00)88942-9.
[Article in French]

Abstract

In order to evaluate the feasibility of the autologous transfusion in an alloimmunized sickle cell patient, changes in the hematologic and biochemical characteristics of erythrocytes stored for 42 days from two patients with sickle cell SC anemia were compared with control subjects' (Hb A) red blood cells. Erythrocytes were stored in Saline Adenosine Dextrose Mannitol at +4 degrees C. The cryopreservation storage was made and 51Cr red cell survival was measured in one patient. No significant difference in the hematologic and biochemical parameters of the SC red blood cells and the control subjects was observed during the storage at +4 degrees C. Red cell survivals determined in fresh cells, cells stored for 42 days at +4 degrees C and thawed cells from one patient demonstrate much shorter half-life values than those of normal red blood cells. Before application, our results need to be confirmed by the same protocol with another patient with sickle cell SC.

Publication types

  • Case Reports
  • Comparative Study
  • English Abstract

MeSH terms

  • Adenosine Triphosphate / blood
  • Adult
  • Anemia, Sickle Cell / genetics*
  • Anemia, Sickle Cell / therapy*
  • Blood Preservation / methods*
  • Blood Transfusion, Autologous*
  • Cryopreservation / methods*
  • Enzymes / blood
  • Erythrocyte Transfusion*
  • Erythrocytes* / physiology
  • Female
  • Heterozygote
  • Humans
  • Male
  • Reference Values
  • Solutions
  • Time Factors

Substances

  • Enzymes
  • Solutions
  • Adenosine Triphosphate