Linear relation between cerebral phosphocreatine concentration and memory capacities during permanent brain vessel occlusions in rats

Ann N Y Acad Sci. 2000 Apr:903:299-306. doi: 10.1111/j.1749-6632.2000.tb06380.x.

Abstract

The present study investigates the interrelation between cerebral energy state and memory capacities in a rat model of stepwise cerebral vessel occlusions. After acute and subchronic permanent vessel occlusions, cortical energy metabolites (ATP, phosphocreatine, ADP, AMP) were detected by high-pressure liquid chromatography (HPLC) analysis, and the effects on learning, memory, and cognitive behavior were evaluated using a hole-board test. The results of the study demonstrated a drastic decrease in energy-rich phosphates by 33% for phosphocreatine and by 44% for ATP after acute vessel occlusions. In addition, rat working and reference memories were strikingly decreased to about 5% of controls. In contrast, two weeks after four-vessel occlusion, the energy state was almost completely restored to control levels. However, a significant decrease in memory capacities was observed in subchronic state. In summary, this study has demonstrated a close linear relationship (p < 0.001) between an impaired cerebral energy state and brain memory dysfunction after acute and permanent cerebral four-vessel occlusion. Thus, this animal model of stepwise reduction of the cerebral blood supply may reflect some clinically relevant processes occurring during cerebrovascular and neurodegenerative diseases.

MeSH terms

  • Adenosine Diphosphate / metabolism
  • Adenosine Monophosphate / metabolism
  • Adenosine Triphosphate / metabolism
  • Animals
  • Brain / metabolism*
  • Brain Ischemia / metabolism*
  • Brain Ischemia / psychology*
  • Carotid Artery, Common
  • Cerebral Cortex / metabolism
  • Cognition
  • Electrocoagulation
  • Energy Metabolism*
  • Learning
  • Male
  • Phosphocreatine / metabolism*
  • Rats
  • Rats, Wistar
  • Vertebral Artery

Substances

  • Phosphocreatine
  • Adenosine Monophosphate
  • Adenosine Diphosphate
  • Adenosine Triphosphate