Solid phase synthesis of C-terminal peptide amides: development of a new aminoethyl-polystyrene linker on the Multipin solid support

J Pept Sci. 2000 May;6(5):243-50. doi: 10.1002/(SICI)1099-1387(200005)6:5<243::AID-PSC251>3.0.CO;2-4.

Abstract

A new aminoethyl-polystyrene linker, stable at low concentrations of TFA, has been developed for the solid phase synthesis of peptide amides. The described linker is stable under conditions which remove Bu(t) protecting groups (30-50% TFA in DCM) and the desired product can be finally cleaved off the solid support in 95% TFA (5% H2O). Model peptide amides and other N-alkylated peptide amides have been successfully synthesized in good yield and purity.

MeSH terms

  • Amides / chemical synthesis*
  • Chromatography, High Pressure Liquid
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Models, Chemical
  • Peptides / chemical synthesis*
  • Polystyrenes / chemistry*
  • Temperature
  • Time Factors

Substances

  • Amides
  • Peptides
  • Polystyrenes