SCF, IL-1beta, IL-1ra and GM-CSF in the bone marrow and serum of normal individuals and of AML and CML patients

Cytokine. 2000 Jun;12(6):699-707. doi: 10.1006/cyto.2000.0666.

Abstract

This study compared cytokine transcript and protein levels in BM cells of normal individuals and leukemic patients. AML differed from normal in that: (1) AML marrow cells contain more IL-1beta protein than normal cells, (2) IL-1ra transcripts are absent from AML marrow cells, (3) AML marrow serum contains less IL-1ra protein than normal, (4) peripheral blood and marrow serum of AML patireents contains more SCF protein than normal serum, and (5) SCF transcripts have been detected in AML marrow biopsies and not in aspirate cells. These data suggest that unbalanced cytokine production may make a significant contribution to the abnormal behaviour of AML cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biopsy
  • Bone Marrow Cells / immunology
  • Bone Marrow Cells / metabolism*
  • Bone Marrow Cells / pathology
  • Granulocyte-Macrophage Colony-Stimulating Factor / blood
  • Granulocyte-Macrophage Colony-Stimulating Factor / genetics*
  • Humans
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1 / blood
  • Interleukin-1 / genetics*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / blood
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
  • Leukemia, Myeloid, Acute / blood
  • Leukemia, Myeloid, Acute / genetics*
  • Reference Values
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sialoglycoproteins / blood
  • Sialoglycoproteins / genetics*
  • Stem Cell Factor / blood
  • Stem Cell Factor / genetics*
  • Transcription, Genetic*

Substances

  • IL1RN protein, human
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1
  • Sialoglycoproteins
  • Stem Cell Factor
  • Granulocyte-Macrophage Colony-Stimulating Factor