A polymorphism upstream from the human paraoxonase (PON1) gene and its association with PON1 expression

Atherosclerosis. 2000 Jun;150(2):295-8. doi: 10.1016/s0021-9150(99)00379-2.

Abstract

Human serum paraoxonase (PON1) is an esterase that has been shown to decrease the susceptibility of lipoproteins to lipid peroxidation. We found a polymorphism of cytosine/thymidine (-108C/T, the number is from the ATG codon) in the upstream region of the PON1 gene. The luciferase activity was lower in the -108T allele than in the -108C allele. The serum PON1 concentrations in 132 normal subjects were as follows: -108CC>-108CT and>-108TT genotypes. The polymorphism upstream from the PON1 gene is associated with transcription of the PON1 gene and the serum PON1 concentration.

Publication types

  • Comparative Study

MeSH terms

  • Alleles
  • Aryldialkylphosphatase
  • Cholesterol, HDL / blood
  • Cholesterol, LDL / blood
  • Coronary Disease / blood
  • Coronary Disease / enzymology
  • Coronary Disease / genetics
  • DNA / analysis*
  • DNA Primers / chemistry
  • Esterases / blood*
  • Esterases / genetics*
  • Female
  • Gene Frequency
  • Genetic Markers
  • Genotype
  • Humans
  • Lipid Peroxidation / genetics*
  • Luciferases / blood
  • Male
  • Middle Aged
  • Polymorphism, Genetic*
  • Reference Values
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription, Genetic / genetics
  • Up-Regulation / genetics

Substances

  • Cholesterol, HDL
  • Cholesterol, LDL
  • DNA Primers
  • Genetic Markers
  • DNA
  • Luciferases
  • Esterases
  • Aryldialkylphosphatase
  • PON1 protein, human