Predicted exocyclic amino group alkylation of 2'-deoxyadenosine and 2'-deoxyguanosine by the isopropyl cation

Chem Res Toxicol. 2000 Jun;13(6):431-5. doi: 10.1021/tx000059j.

Abstract

Diisopropyltriazene in aqueous 10% acetonitrile (pH 7.0 +/- 0.4) yields N(6)-isopropyl-2'-deoxyAdo as the predominant product and N(2)-isopropyl-2'-deoxyGuo in yields comparable with the O(6) adduct in reactions with 2'-deoxyAdo and 2'-deoxyGuo, respectively. These observations are inconsistent with what is expected on the basis of the regnant hypothesis concerning factors that determine atom site selectivity in diazonium ion-mediated alkylations. An alternative explanation based on the fleeting existence of the reactive intermediates involved is consistent with these observations.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alkylation
  • Amines / chemistry
  • Cations
  • DNA Adducts / chemistry*
  • Deoxyadenosines / chemistry*
  • Deoxyguanosine / chemistry*
  • Propanols / chemistry*

Substances

  • Amines
  • Cations
  • DNA Adducts
  • Deoxyadenosines
  • Propanols
  • Deoxyguanosine
  • 2'-deoxyadenosine