Abstract
Noninflamed skin venules support constitutive leukocyte rolling. P-selectin controls the rolling frequency, whereas E-selectin dictates rolling velocity (Vroll). Fucosylated selectin ligands are essential for all interactions, as rolling was absent in mice doubly deficient in alpha1,3-fucosyltransferase (FucT)-IV and FucT-VII. The rolling fraction was reduced in FucT-VII-/- animals but normal in FucT-IV-/- mice. However, Vroll was markedly increased in both strains. P-selectin ligands generated by FucT-VII are crucial for initial leukocyte tethering, whereas E-selectin ligands that permit maximum slowing of Vroll require simultaneous expression of FucT-IV and FucT-VII. These results demonstrate a role for FucT-IV in selectin-dependent adhesion and suggest that the endothelial selectins and FucTs have distinct but overlapping functions in the immunosurveillance of the skin.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Movement / genetics
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Cell Movement / immunology*
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E-Selectin / biosynthesis
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E-Selectin / metabolism
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E-Selectin / physiology
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Ear / blood supply
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Female
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Fucosyltransferases / metabolism
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Fucosyltransferases / physiology*
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Inflammation / enzymology
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Inflammation / immunology
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L-Selectin / genetics
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L-Selectin / physiology
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Leukocytes / enzymology*
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Leukocytes / immunology*
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Leukocytes / metabolism
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Ligands
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Microcirculation / enzymology
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Microcirculation / immunology
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Microcirculation / metabolism
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P-Selectin / genetics
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P-Selectin / metabolism
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P-Selectin / physiology
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Skin / blood supply*
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Skin / enzymology
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Skin / immunology
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Skin / pathology
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Venules / enzymology
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Venules / immunology
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Venules / metabolism
Substances
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E-Selectin
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Ligands
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P-Selectin
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L-Selectin
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Fucosyltransferases
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galactoside 3-fucosyltransferase