Abstract
In the present work we report the development of a new radioimmunoassay method for measuring the substance P content liberated from isolated rat tracheae in response to electrical or chemical (capsaicin, resiniferatoxin, piperine) stimulation. The amount of substance P released by electrical stimulation has been found to be dependent on the number of pulses and chemically elicited substance P release also proved to be dose-dependent. Our findings reinforce previous data that resiniferatoxin is approximately 100 times more potent than capsaicin and the potency ratio between piperine and capsaicin is 1/50.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaloids*
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Animals
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Benzodioxoles
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Calibration
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Capsaicin / pharmacology
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Diterpenes / pharmacology
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Electric Stimulation
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Enzyme Inhibitors / pharmacology
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Female
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Neurons, Afferent / drug effects
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Neurons, Afferent / metabolism*
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Piperidines / pharmacology
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Polyunsaturated Alkamides
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Radioimmunoassay / methods*
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Rats
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Rats, Wistar
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Stimulation, Chemical
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Substance P / analysis*
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Substance P / metabolism*
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Trachea / innervation*
Substances
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Alkaloids
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Benzodioxoles
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Diterpenes
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Enzyme Inhibitors
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Piperidines
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Polyunsaturated Alkamides
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Substance P
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resiniferatoxin
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Capsaicin
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piperine