Abstract
From genomic DNA of Ralstonia pickettii isolate PIC-1, a beta-lactamase gene was cloned that encodes the oxacillinase OXA-22. It differs from known oxacillinases, being most closely related to OXA-9 (38% amino acid identity). The hydrolytic spectrum of OXA-22 is limited mostly to benzylpenicillin, cloxacillin, and restricted-spectrum cephalosporins. OXA-22-like genes were identified as single chromosomal copies in five other R. pickettii clinical isolates. The expression of OXA-22-like beta-lactamases was inducible in R. pickettii.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Anti-Bacterial Agents / pharmacology
-
Chromosomes, Bacterial
-
Cloning, Molecular
-
Enzyme Induction
-
Humans
-
Microbial Sensitivity Tests
-
Molecular Sequence Data
-
Pseudomonas / drug effects
-
Pseudomonas / enzymology
-
Pseudomonas / genetics*
-
Pseudomonas / metabolism
-
Sequence Homology, Amino Acid
-
beta-Lactam Resistance / genetics
-
beta-Lactamases / biosynthesis
-
beta-Lactamases / genetics*
-
beta-Lactams
Substances
-
Anti-Bacterial Agents
-
beta-Lactams
-
beta-lactamase OXA-22
-
beta-Lactamases