Abstract
We identified a novel missense mutation in the myophosphorylase gene (PYGM) in a Spanish patient with McArdle's disease. This homozygous C-to-A mutation results in the replacement of a highly conserved alanine at amino acid position 659 with an aspartic acid in the C-terminal domain of the myophosphorylase gene protein, near binding sites for pyridoxal phosphate and glucose. Our data further expand the genetic heterogeneity in patients with McArdle's disease.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Binding Sites / genetics
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Biopsy
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Conserved Sequence
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DNA Mutational Analysis
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Glycogen Storage Disease Type V / diagnosis*
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Glycogen Storage Disease Type V / genetics*
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Homozygote*
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Humans
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Male
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Muscle, Skeletal / enzymology
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Muscle, Skeletal / pathology
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Mutation, Missense / genetics*
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Phosphorylases / genetics*
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Phosphorylases / metabolism
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length
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Spain